1.
Click on "Play".
2.
Click on the arrow to proceed through the introduction to Koch's methods.
3.
Click on the door to enter Koch's house.
4.
To carry out the first of Koch's methods -- drying or fixing bacteria onto glass slides -- do the following:
a) Pick up the bottles of methylene blue dye and vesuvian brown dye from the table by moving the mouse cursor on top of them and clicking on the "hand symbol" that appears. These and subsequent items that you pick up will be stored in your inventory, which you can access at any time by clicking the "Inventory" button in the upper right-hand corner of the game window.
b) Pick up the glass slides on the table in the same manner as before.
c) Click on the door to the left of the cages to allow Wilma to enter another room.
5.
Pick up the expectorant sample on top of the shelf. Click on the expectorant sample in the inventory and move the cursor to the top of the slide as shown above. Click again to release the sample, which places it on the slide.
6.
After clicking anywhere in the game window, you can read two short pages about Koch's slide technique. Go forward by clicking on the arrows. Click on the "Back to game" button on the last page to carry out the second of Koch's methods - staining.
7.
To stain your bacteria sample, do the following:
a) Click on the methylene blue dye in the inventory and drag it on top of the slide.
Click again to release the dye, which places it on the slide.
b) Do the same thing with the vesuvian brown dye. Click on it and drag it on top of the slide. Click again to release the dye, which places it on the slide.
8.
After clicking anywhere in the game window, you can read four short pages about Koch's staining technique. Use the orange arrows to go forward and then click on the "Back to game" button to view bacteria under the microscope.
9.
Go back to the room with the microscope by moving the mouse cursor onto the door and clicking on the hand symbol. Click on the slide in the inventory and drag it on top of the microscope. Click again to release the sample, which places it under the microscope.
10.
You will now be able to look at the bacteria under the microscope.
a)
Drag the cursor on the knob on the left to focus.
b) Drag the cursor on the arrows on the right to study the bacteria.
c) Click on the "Continue" button on the last text page when you are ready to move on and test the bacteria to see if they actually cause Tb - the third of Koch's postulates.
11.
To test your bacteria sample, do the following:
Click on the expectorant sample in the inventory and drag it on top of the culture plate on the table.
Click again to release the sample, which places it on the culture plate.
After clicking anywhere in the game window, you can read about Koch's plate technique on a few text pages. Click on the "Back to game" button on the last text page when you are ready to move on.
12.
Now you can enter the door to the left of the sink.
13.
Click anywhere on the green ground.
14.
Catch the guinea pigs by clicking on them with the mouse cursor. You need to catch eight in total to move on.
15.
After reading Koch's Postulates, click on "Continue". After a few more text pages you can continue by clicking on the "Back to game" button.
16.
To test your bacteria sample, do the following:
a) Pick up the syringe on the table.
b) Click on the syringe in the inventory and drag it on top of the culture plate on the table.
c)
Click again to fill the syringe with a sample from the culture plate.
d) Click on the syringe in the inventory once more and drag it on top of a guinea pig in one of the cages. Click again to inject the guinea pig with the contents of the syringe.